THE DIFFUSION COEFFICIENT OF CRYSTALLINE TRYPSIN
نویسندگان
چکیده
منابع مشابه
The Diffusion Coefficient of Crystalline Trypsin
The diffusion coefficient of crystalline trypsin in 0.5 saturated magnesium sulfate at 5 degrees C. is 0.020 +/-0.001 cm.(2) per day, corresponding to a molecular radius of 2.6 x 10(-7) cm. The rate of diffusion of the proteolytic activity is the same as that of the protein nitrogen, indicating that these two properties are held together in chemical combination and not in the form of an adsorpt...
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A method is described for isolating a crystalline protein of high tryptic activity from beef pancreas. The protein has constant proteolytic activity and optical activity under various conditions and no indication of further fractionation could be obtained. The loss in activity corresponds to the decrease in native protein when the protein is denatured by heat, digested by pepsin, or hydrolyzed ...
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1. The rate of inactivation of crystalline trypsin solutions and the nature of the products formed during the inactivation at various pH at temperatures below 37 degrees C. have been studied. 2. The inactivation may be reversible or irreversible. Reversible inactivation is accompanied by the formation of reversibly denatured protein. This denatured protein exists in equilibrium with the native ...
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A study has been made of the general properties of crystalline soybean trypsin inhibitor. The soy inhibitor is a stable protein of the globulin type of a molecular weight of about 24,000. Its isoelectric point is at pH 4.5. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly...
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The fatty liver that develops in insulin-treated, depancreatized dogs and in dogs subjected to ligation of their pancreatic ducts has been the subject of considerable study (1). Soon after its discovery it was shown to be prevented by the feeding of raw pancreas (2), while later findings revealed that it could also be prevented by the feeding of pancreatic juice and certain fractions derived fr...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1933
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.16.5.795